by Rousseau J., Nakamura M., Rio-Maior H., Álvares F., Choquet R., Madeira de Carvalho L., Godinho R. & Santos N.
Abstract:
Sarcoptic mange is globally enzootic, and non-invasive methods with high diagnostic specificity for its surveillance in wildlife are lacking. We describe the molecular detection of Sar-coptes scabiei in non-invasively collected faecal samples, targeting the 16S rDNA gene. We applied this method to 843 Iberian wolf Canis lupus signatus faecal samples collected in north-western Portugal (2006–2018). We further integrated this with serological data (61 samples from wolf and 20 from red fox Vulpes vulpes, 1997–2019) in multi-event capture–recapture models. The mean predicted prevalence by the molecular analysis of wolf faecal samples from 2006–2018 was 7.2% (CI95 5.0–9.4%; range: 2.6–11.7%), highest in 2009. The mean predicted seroprevalence in wolves was 24.5% (CI95 18.5–30.6%; range: 13.0–55.0%), peaking in 2006–2009. Multi-event capture–recapture models estimated 100% diagnostic specificity and moderate diagnostic sensitivity (30.0%, CI95 14.0– 53.0%) for the molecular method. Mange-infected individually identified wolves showed a tendency for higher mortality versus uninfected wolves (ΔMortality 0.150, CI95 −0.165–0.458). Long-term serology data highlights the endemicity of sarcoptic mange in wild canids but uncovers multi-year epidemics. This study developed and evaluated a novel method for surveying sarcoptic mange in wildlife populations by the molecular detection of S. scabiei in faecal samples, which stands out for its high specificity and non-invasive character.
Reference:
Rousseau J., Nakamura M., Rio-Maior H., Álvares F., Choquet R., Madeira de Carvalho L., Godinho R. & Santos N. (2021) Non-invasive molecular survey of sarcoptic mange in wildlife: Diagnostic performance in wolf faecal samples evaluated by multi-event capture–recapture models. Pathogens. 10: 1-15.
Bibtex Entry:
@article{RousseauEtal2021,
abstract = {Sarcoptic mange is globally enzootic, and non-invasive methods with high diagnostic specificity for its surveillance in wildlife are lacking. We describe the molecular detection of Sar-coptes scabiei in non-invasively collected faecal samples, targeting the 16S rDNA gene. We applied this method to 843 Iberian wolf Canis lupus signatus faecal samples collected in north-western Portugal (2006–2018). We further integrated this with serological data (61 samples from wolf and 20 from red fox Vulpes vulpes, 1997–2019) in multi-event capture–recapture models. The mean predicted prevalence by the molecular analysis of wolf faecal samples from 2006–2018 was 7.2\% (CI95 5.0–9.4\%; range: 2.6–11.7\%), highest in 2009. The mean predicted seroprevalence in wolves was 24.5\% (CI95 18.5–30.6\%; range: 13.0–55.0\%), peaking in 2006–2009. Multi-event capture–recapture models estimated 100\% diagnostic specificity and moderate diagnostic sensitivity (30.0\%, CI95 14.0– 53.0\%) for the molecular method. Mange-infected individually identified wolves showed a tendency for higher mortality versus uninfected wolves (ΔMortality 0.150, CI95 −0.165–0.458). Long-term serology data highlights the endemicity of sarcoptic mange in wild canids but uncovers multi-year epidemics. This study developed and evaluated a novel method for surveying sarcoptic mange in wildlife populations by the molecular detection of S. scabiei in faecal samples, which stands out for its high specificity and non-invasive character.},
author = {Rousseau, Julieta and Nakamura, Mónia and Rio-Maior, Helena and Álvares, Francisco and Choquet, Rémi and Madeira de Carvalho, Luís and Godinho, Raquel and Santos, Nuno},
doi = {10.3390/PATHOGENS10020243},
issn = {20760817},
issue = {2},
journal = {Pathogens},
keywords = {Canis lupus,Epidemiology,Iberian Penin-sula,PCR,Sarcoptes scabiei,Serology,Vulpes vulpes},
month = {2},
pages = {1-15},
title = {Non-invasive molecular survey of sarcoptic mange in wildlife: Diagnostic performance in wolf faecal samples evaluated by multi-event capture–recapture models},
volume = {10},
year = {2021},
}